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Open Access Highly Accessed Research article

Gait analysis in a murine model of collagen-induced arthritis

Jon Vincelette1, Yifan Xu1, Le-Ning Zhang1*, Caralee J Schaefer1, Ronald Vergona1, Mark E Sullivan1, Thomas G Hampton2 and Yi-Xin (Jim) Wang1

Author Affiliations

1 Bayer HealthCare Pharmaceuticals, 800 Dwight Way, Berkeley, CA 94701, USA

2 Mouse Specifics, Inc., 28 State St., Suite 1112, Boston, MA 02109, USA

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Arthritis Research & Therapy 2007, 9:R123  doi:10.1186/ar2331

Published: 24 November 2007

Abstract

Murine collagen-induced arthritis (CIA) has become a valuable animal model for elucidating pathogenic mechanisms and evaluating therapeutic effects for rheumatoid arthritis. Recent advances in digital imaging and computer technology have enabled gait analysis to develop into a powerful tool for objectively detecting functional deficits in human and animal models. The present study explored the use of non-invasive video-capture gait analysis in the evaluation of a murine CIA model. CIA was induced in 45 female DBA/1LacJ mice (8 to 10 weeks old) by immunization with lyophilized bovine articular type II collagen. Gait parameters were determined by ventral plane videography and were correlated to traditional arthritis clinical scores. Our results showed that increases in clinical scores that measure the severity of CIA corresponded to changes in multiple gait parameters that reflect both morphologic (increases in paw area) and functional (increase in stride frequency, decrease in stride length, hind-limb paw placement angle, as well as stride, stance, and braking times) deficits. Our work indicated that the non-invasive video-capture device may be used as a simple and objective data acquisition system for quantifying gait disturbances in CIA mice for the investigation of mechanisms and the evaluation of therapeutic agents.