Table 3

Summary of effects of the root extracts*

Parameter

Assay

Change

No. of mice per group


Leukocyte count, exudate

Cell count

-87%

10

Neutrophil density, membrane

Cell count

-68%

4

IL-6 protein, exudate

ELISA

-50%

7

IL-6 mRNA, membrane

qRT-PCR

-100%

4 + 4**

TNF-α mRNA, membrane

qRT-PCR

-100%

4 + 4**

PGE2, exudate

ELISA

-69%

7

PGD2, exudate

ELISA

+5.2-fold

7

Ratio of PGD2:PGE2

ELISA

+9.0-fold

7

h-PGDS mRNA, membrane

qRT-PCR

+3.7-fold

5


* Compared with MSU-stimulated pouches from mice fed water. All percentage differences were significant at p < 0.05.

**Duplicate experiments. ELISA, enzyme-linked immunosorbent assay; h-PGDS, hematopoietic prostaglandin D synthase; IL, interleukin; MSU, monosodium urate; PGD2, prostaglandin D2; PGE2, prostaglandin E2; qRT-PCR, relative quantitative reverse transcriptase polymerase chain reaction; TNF, tumor necrosis factor.

Jung et al. Arthritis Research & Therapy 2007 9:R64   doi:10.1186/ar2222

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