Table 1 |
||||
|
PCR primer sequences |
||||
|
Gene product |
Sequences |
Accession number |
Position |
Product (base-pairs) |
|
|
||||
|
Collagen I(a2) |
Up: 5'-CTGGTCCTTCTGGTCCTGTTG |
NM_000089 |
3,413 |
|
|
Low: 5'-GTGCGAGCTGGGTTCTTTCTA |
3,957 |
544 |
||
|
Collagen II(a1) |
Up: 5'-CTGGCTCCCAACACTGCCAACGTC |
NM_033150 |
4,070 |
|
|
Low: 5'-TCCTTTGGGTTTGCAACGGATTGT |
4,483 |
413 |
||
|
Collagen X |
Up: 5'-ACCCAAGAGGTGCCCCTGGAATAC |
NM_000493.2 |
1,416 |
|
|
Low: 5'-CCTGAGAAAGAGGAGTGGACATAC |
2,117 |
701 |
||
|
Aggrecan |
Up:5'-AGCTGGGTTCGGGGCATCT |
NM_013227 |
6,039 |
|
|
Low:5'-TGGTAGTCTTGGGCATTGTTGTTGA |
6,839 |
800 |
||
|
IL-1β |
Up:5'-ATGGCAGAAGTACCTAAGCTCGC |
NM_000576 |
87 |
|
|
Low:5'-ACACAAATTGCATGGTGAAGTCAGTT |
889 |
802 |
||
|
ALK-1 |
Up: 5'-CGGCTCCCTCTACGACTTTCT |
Z_22533 |
1,128 |
|
|
Low: 5'-CAGCACTCCCGCATCATCT |
1,479 |
570 |
||
|
GAPDH |
Up: 5'-TGAAGGTCGGAGTCAACGGATTTGGT |
NM_002046 |
113 |
|
|
Low: 5'-CATGTGGGCCATGAGGTCCACCAC |
1,095 |
983 |
||
|
|
||||
|
ALK = activin-like kinase; GAPDH = glyceraldehyde-3-phosphate dehydrogenase. |
||||
|
Stoop et al. Arthritis Research & Therapy 2007 9:R60 doi:10.1186/ar2218 |
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