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DNA sequences of the forward and reverse primers for the qualitative and semi-quantitative reverse transcriptase-polymerase chain reaction analyses |
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| Gene identity |
Accession number |
Primer sequencesa |
Annealing temperature (°C) |
Number of cycles (Quan.)b |
Number of cycles (Qual.)c |
Size of PCR product (bp) |
| RANK-L |
AF019047 |
5'-CTG-ATG-AAA-GGA-GGA-AGC-AC-3' |
65 |
29 |
35 |
546 |
| 5'-GAT-GAC-ACC-CTC-TCC-ACT-TC-3' |
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| OPG |
U94332 |
5'-TGC-TGT-TCC-TAC-AAA-GTT-TAC-G-3' |
56 |
35 |
40 |
433 |
| 5'-CTT-TGA-GTG-CTT-TAG-TGC-GTG-3' |
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| RANK |
AF018253 |
5'-CCT-GGA-CCA-ACT-GTA-CCT-TC-3' |
58 |
34 |
40 |
500 |
| 5'-TTC-CTC-TAT-CTC-GGT-CTT-GC-3' |
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| TRAF6 |
U78798 |
5'-TGA-TAG-TGT-GGG-TGG-AAC-TG-3' |
58 |
27 |
35 |
456 |
| 5'-CTC-CTT-GGA-CAA-TCC-TTC-AG-3' |
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| β-Actin |
NM001101 |
5'-CGT-GAC-ATT-AAG-GAG-AAG-CTG-TGC-3' |
58 |
21 |
28 |
375 |
| 5'-CTC-AGG-AGG-AGC-AAT-GAT-CTT-GAT-3' |
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aFor each pair of sequences, the forward primer appears first and the reverse primer appears second. bThe number of cycles used in the semi-quantitative (quan.) PCR experiments corresponds to the linear phase of the amplification reaction. cThe number of cycles used in the qualitative (qual.) PCR experiments was increased to allow the possible detection of the mRNA. OPG, osteoprotegerin; PCR, polymerase chain reaction; RANK, receptor activator of nuclear factor-kappa-B; RANK-L, ligand of receptor activator of nuclear factor-kappa-B; TRAF6, tumor necrosis factor receptor-associated factor 6. | ||||||
Poubelle et al. Arthritis Research & Therapy 2007 9:R25 doi:10.1186/ar2137 |
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