Figure 2.

Investigation of the selective accumulation of the small immunoproteins L19 and G11 in inflamed limbs of arthritic mice. Arthritic mice were injected with SIP(L19)–Alexa750 (Molecular Probes, Leiden, The Netherlands), SIP(G11)–Alexa750 or the control antibody SIP(HyHEL10)–Alexa750. Near-infrared fluorescence imaging analysis was performed 24 hours after injection of the fluorescence-labelled antibodies. Arrows indicate grade 2 swelling in the front paws of the mice. In a similar experiment, arthritic mice were injected with ¹²⁵I-labelled SIP(L19), SIP(G11) or SIP control. Uptake of radio-iodinated antibodies was analysed by phosphorimaging 24 hours after injection. The mouse injected with ¹²⁵I-labelled SIP(L19) had an arthritic score of 2 in the left paw, whereas the right paw was classified as grade 1 arthritis. In the mouse injected with ¹²⁵I-labelled SIP(G11) the left paw was classified as grade 1 arhritis, whereas the right paw was classified as grade 2 arthritis. In the mouse injected with ¹²⁵I-labelled SIP(F16) the left paw was classified as grade 2 arthritis, whereas the right paw as grade 1 arthritis.