Figure 4.

Influence of fibroblast-like synovial (FLS) cells on bradykinin 2 (B2) receptor-like labelling in dorsal root ganglion (DRG) neurones. (a) Co-culture of DRG neurones and FLS cells isolated after induction of acute antigen-induced arthritis (AIA). Dark DRG neurones (arrows) show bradykinin-gold labelling. (b) Same cells as in (a) double-labelled with an anti-neurone-specific enolase antibody. Scale bar, 10 μm. (c) Influence of FLS cells isolated from normal, acutely or chronically inflamed knees on B2 receptor-like labelling in DRG neurones. ^#Significant difference to DRG mono-cultures. ^$Significant difference to co-culture with FLS cells from normal knee joints. AIA, antigen-induced arthritis.