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Genes whose expression showed the greatest change following incubation of chondrocytes with elevated IL-1β alone: genes of known function whose expression was most affected by IL-1 alone |
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| Genes whose RNA levels were reduced >80% relative to untreated control cultures |
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| • A disintegrin and metaloproteinase domain 33 |
• c-fos-induced growth factor (vascular endothelial growth factor D) |
Kruppel associated box zinc finger 1 |
• Smoothelin |
| • Actin alpha 1 |
• Collagen type II alpha 1, chain precursor |
• Microfibril-associated glycoprotein precursor |
Solute carrier family 39 (iron-regulated transporter), member 1 |
| Aggrecan 1 |
Crystallin, alpha B |
• Midline 1 |
• Sphingomyelin phosphodiesterase 3, neutral |
| • Ankyrin-like repeat protein |
Distal-less homeobox |
• Mitochondrial ribosomal protein L53 |
• Transforming growth factor, beta 2 |
| • Annexin III (Lipocortin III) |
• DVS27-related protein |
Myocilin precursor |
• Zinc finger protein SLUG (neutral crest transcription factor Slug) |
| Cadherin-8 precursor |
• Dynein, cytoplasmic, intermediate chain 1 |
• NAD-dependent 15-hydroxyprostaglandin dehydrogenase |
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| Calpain 6 |
• Enolase 3, beta |
Osteomodulin (osteoadherin) |
|
| • Carbonic anhydrase 3 |
Four and a half LIM domains 1 |
• Palmdelphina |
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| Cartilage link protein 1 a |
• Heat shock protein HSP 90 betaa |
• Phosphoribosyl pyrophosphate synthestase 2 |
|
| • Caveolin 3 |
Insulin-like growth factor binding protein 4 precursor |
• Programmed cell death protein 7 |
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| Genes whose RNA levels were increased more than fivefold relative to untreated controls |
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| Adaptor protein with pleckstrin homology and src homology 2 domains |
Cyclooxygenase-2 |
Keratin, type II cryosqueletal 8 |
Plasminogen activator, urokinase receptor |
| Adenosine A2B receptor |
Cytochrome P450, family 26, subfamily b, polypeptide 1 |
Lactose operon repressor |
Prostaglandin E synthase a |
| Adenosine monophosphate deaminase 3 |
Cytochrome P450, subfamily 7B, polypeptide 1a |
Laminin beta-2 chain precursor |
Purigenic receptor P2Y, G-protein coupled 2 |
| Aldose reductase-like protein |
Cytokine-induced neutrophil chemoattractant-2a |
MAP-kinase phosphatase (cpg21) |
RAB27B, member RAS oncogene family |
| Apoptotic death agonist BID |
EGL nine homolog 3 (C. elegans) |
Matrix metalloproteinase 3 |
Rat VL30 element a |
| Arginosuccinate synthetase |
Endothelial cell-specific molecule 1 |
Matrix metalloproteinase 9a |
Receptor-interacting serine-threonine kinase 2 |
| ATP-binding cassette, sub-family G (WHITE), member 1 |
Epiregulin precursor |
Matrix metalloproteinase 10 |
RelB |
| BCL2-related protein A1 |
Fatty acid binding protein 4 |
Matrix metalloproteinase 12 |
Retinol-binding protein 1 |
| Beta-nerve growth factor |
F-box protein Fbx5 |
• Matrix metalloproteinase 13 |
• Ring finger protein 28 |
| Bloom's syndrome protein homolog (mBLM) |
Fibroblast growth factor 9 |
Mesothelin |
Schlafen 4 |
| Bone morphogenetic protein 2a |
Follistatin |
Microtubule-associated protein |
Small inducible cytokine A2 |
| Bone morphogenetic protein 6a |
Fos-like antigen 1 |
6 a |
Smhs1 protein |
| Bone morphogenetic protein 7 |
Gardner-Rasheed feline sarcoma viral (Fgr) oncogen homolog |
Mitochondrial solute carrier protein |
Solute carrier family 1, member 1 |
| Brain-specific angiogenesis inhibitor 1-associated protein 2 |
GATA-binding protein 2 |
Myotubularin related protein 7 |
Solute carrier family 1, member 3 |
| CCL3 (Mip-1a) |
Gro |
NADH-ubiquinone oxidoreductase MLRQ subunit |
Solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 |
| CCL5 (RANTES) |
Growth arrest specific 7 |
Neurofilament, heavy polypeptide |
Solute carrier family 11, member 2 |
| CCL7 (MCP-3) |
GTP cyclohydrolase 1 |
Neuron specific protein PEP-19 (Purkinje cell protein 4) |
Solute carrier family 20 (phosphate transporter), member 1 |
| CD44 antigen |
High mobility group AT-hook 1 |
Neuropeptide Y |
Superoxide dismutase 2 |
| Chemokine receptor (LCR1)a |
IκB-α |
Neurospin precursor |
T-cell death associated gene |
| Cholesterol 15-hydroxylase |
IκB-β (nuclear factor kappa B p105 subunit) |
Nitric oxide synthase 2 |
Testis-specific protein Bs13 |
| Claudin-3 |
Inhibin beta-A |
Organic cation transporter OCTN1 |
Tissue factor pathway inhibitor 2 |
| Colony stimulating factor 2 (granulocyte-macrophage) |
Inhibitor of apoptosis protein 1 |
Parvalbumin |
Tissue-type transglutaminase |
| Colony stimulating factor 3 |
Insulin-like growth factor-binding protein 5 |
Phosphodiesterase 4B, cAMP-specific (dunce (Drosophila) homolog phosphodiesterase E4) |
Toll-like receptor 2 |
| Complement factor B precursor (C3/C5 convertase) |
Interleukin 1 alpha |
Phospholipase A2, group 5 |
Tumor necrosis factor alpha-induced protein 2a |
| CXCL1 (GROa) |
Interleukin 1 beta |
Phospholipase A2, group IIA (platelets, synovial fluid) |
Tumor necrosis factor receptor superfamily member 5 precursor |
| CXCL2 (GROb) |
Interleukin 6 (interferon, beta 2) |
Phospholipid scramblase 1 |
Tumor necrosis factor receptor superfamily member 9 precursor |
| CXCL5 (LIX) |
Interleukin 23, alpha subunit p19 |
Plasminogen activator inhibitor 2 type A |
Uridine phosphorylase |
| CXCL10 (IP-10) |
• Interleukin 13 receptor, alpha 2 |
Plasminogen activator, tissue |
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aRepresents multiple probe sets. Bold circles indicate genes for which the IL-1-mediated effects on transcription were not inhibited by exogenous glucosamine (see text). | |||
Gouze et al. Arthritis Research & Therapy 2006 8:R173 doi:10.1186/ar2082 |
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