Table 1 |
|
|
Primer sequences used to quantify gene expression with real-time PCR |
|
| Gene |
Sequence (5' to 3') |
|
|
|
| Cartilage link protein |
Forward, GCATCAAGTGGACCAAGCTA |
| Reverse, GTAACTCCAATGCCACCACA |
|
| Collagen alpha 1 type II |
Forward, GTGGAGCAGCAAGAGCAAGGA |
| Reverse, CTTGCCCCACTTACCAGTGTG |
|
| CXCL5 (LIX) |
Forward, CACCCTGCTGGCATTTCTG |
| Reverse, AACCATGGCCGAGAAAGGA |
|
| MMP-3 |
Forward, CTGGAATGGTCTTGGCTCAT |
| Reverse, CTGACTGCATCGAAGGACAA |
|
| MMP-9 |
Forward, CCACCGAGCTATCCACTCAT |
| Reverse, GTCCGGTTTCAGCATGTTTT |
|
| MMP-12 |
Forward, TGCAGCTGTCTTTGATCCAC |
| Reverse, TCCAATTGGTAGGCTCCTTG |
|
| TIMP-3 |
Forward, GTACACAGGGCTGTGCAACTTTGTG |
| Reverse, CTTCTGCCGGATGCAGGCGTAGTG |
|
| EF1alpha |
Forward, GATGGCCCCAAATTCTTGAAG |
| Reverse, GGACCATGTCAACAATGGCAG |
|
|
|
|
|
Gouze et al. Arthritis Research & Therapy 2006 8:R173 doi:10.1186/ar2082 |
|
