Figure 4.

The phosphatidyl-inositol-3-kinase pathway regulates both NFκB-dependent and NFκB-independent contact-dependent chemokine production. Macrophage-colony stimulating factor-differentiated monocytes were preincubated for 30 minutes in the presence or absence of variable amounts of LY294002 (as shown) before being stimulated with anti-CD3-activated T cells (Ttcr) or cytokine-activated T cells (Tck) at a T cell:monocyte ratio of 7:1. After 18 hours, culture supernatants were isolated and levels of (a) macrophage inflammatory protein 1 alpha (MIP-1α) (CC chemokine) and (b) interferon-gamma-inducible protein (IP-10) (CXC chemokine) were measured by ELISA. Data represent the mean of triplicate cultures ± standard deviation and are representative of at least three experiments.