Figure 4.

Apoptosis induced by PUMA in p53^+/+ and p53^-/- murine fibroblast-like synoviocytes (FLS). Murine p53^+/+ and p53^-/- FLS at passage 6 were transfected as described in the Materials and methods section. Cell viability by trypan blue staining (a) (*p < 0.05, n = 3) and histone release (b) (*p < 0.05, n = 3) was used to evaluate PUMA-induced apoptosis in both wild-type and p53-knockout FLS.