Table 1 |
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|
DR4 T-cell response to analog peptides after immunization with type II collagen (CII) |
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| Peptide |
Sequencea 263 266 |
IFN-γ (pg/ml) |
IL-10 (pg/ml) |
IL-4 (pg/ml) |
|
|
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| Wild type |
G-E-B-G-I-A-G-F-K-G-E-Q-G-P-K-G-E-B-G-P-A |
1,575 ± 20 |
570 ± 8 |
12 ± 3 |
| A9 |
- - - - A B - N - - - - - - - - - - - - - |
350 ± 11 |
580 ± 11 |
33 ± 12 |
| A12 |
- - - - - - - N - - D - - - - - - - - - - |
175 ± 7 |
610 ± 7 |
48 ± 15 |
| A13 |
- - - - - - - N - - A - - - - - - - - - - |
<1 |
575 ± 9 |
66 ± 10 |
| 263N |
- - - - - - - N - - - - - - - - - - - - - |
263 ± 13 |
560 ± 13 |
9 ± 9 |
| 266A |
- - - - - - - - - - A - - - - - - - - - - |
<1 |
590 ± 10 |
6 ± 9 |
| 266D |
- - - - - - - - - - D - - - - - - - - - - |
175 ± 6 |
600 ± 12 |
9 ± 5 |
|
|
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|
Pooled splenocytes and lymph node cells (5 × 106 cells/ml) from DR4 mice immunized with CII 10 to 14 days previously were cultured with 100 μg/ml antigen (synthetic peptides). Each experiment included cells from three mice and was performed three times. Supernatants were collected and assayed for IFN-γ, IL-4, and IL-10. Values are means ± SD. Numbers in bold type indicate values greater than fivefold those of wild-type peptide. The cytokine response to wild-type peptide included primarily IFN-γ and IL-10. In contrast, the analog peptides A12 and A13 induced the production of predominantly IL-4 and IL-10. |
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|
Sakurai et al. Arthritis Research & Therapy 2006 8:R150 doi:10.1186/ar2043 |
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