Table 1 |
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Bovine metalloproteinase and inhibitor primers for real-time polymerase chain reaction |
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Gene |
Sequence (5'-3') |
Length (bp) |
|
|
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|
MMP-1 |
GATGCCGCTGTTTCTGAGGA |
372 |
|
GACTGAGCGACTAACACGACACAT |
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MMP-2 |
TCTGCCCCCATGAAGCCCTGTT |
347 |
|
GCCCCACTTGCGGTCATCATCGTA |
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|
MMP-3 |
TTAGAGAACATGGGGACTTTTTG |
360 |
|
CGGGTTCGGGAGGCACAG |
||
|
MMP-8 |
ATGCTGCTTATGAGGATTTTGACA |
101 |
|
GCCTGGGGTAACCTTGCTGAGTA |
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|
MMP-9 |
CGCCACCACCGCCAACTACG |
350 |
|
GGGGGTGCTCCTCTGTGAATCTGT |
||
|
MMP-12 |
TGTGACCCCAATATGAGTTTT |
155 |
|
TTGAATGTAAGACGGTAGGTTT |
||
|
MMP-13 |
CCCTCTGGTCTGTTGGCTCAC |
304 |
|
CTGGCGTTTTGGGATGTTTAGA |
||
|
MMP-14 |
AGGCCGACATCATGATCTTCTTTG |
375 |
|
CTGGGTTGAGGGGGCATCTTAGTG |
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|
MMP-16 |
ACCCCAGGATGTCAGTGC |
287 |
|
AATAGCTTTACGGGTTTCAGG |
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|
TIMP-1 |
TGGGCACCTGCACATCACC |
277 |
|
CATCTGGGCCCGCAAGGACTG |
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|
TIMP-2 |
ATAGTGATCAGGGCCAAAGCAGTC |
277 |
|
TGTCCCAGGGCACGATGAAGTC |
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|
TIMP-3 |
GATGTACCGAGGATTCACCAAGAT |
356 |
|
GCCGGATGCAAGCGTAGT |
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|
TIMP-4 |
ATATTTATACGCCTTTTGATTCCT |
297 |
|
GGTACCCGTAGAGCTTCCGTTCC |
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|
α2M |
GCCCGCTTTGCCCCTAACA |
359 |
|
TCGTCCACCCCACCCTTGATG |
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|
RECK |
GTAATTGCCAAAAAGTGAAA |
352 |
|
TAGGTGCATATAAACAAGAAGTA |
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|
ADAMTS-1 |
GCTGCCCTCACACTGCGGAAC |
264 |
|
CATCATGGGGCATGTTAAACAC |
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ADAMTS-4 |
GCGCCCGCTTCATCACTG |
101 |
|
TTGCCGGGGAAGGTCACG |
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ADAMTS-5 |
AAGCTGCCGGCCGTGGAAGGAA |
196 |
|
TGGGTTATTGCAGTGGCGGTAGG |
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|
ADAMTS-8 |
AGATCTTTGGGCTGGGCTTCC |
116 |
|
GGCTGGCATTCCTCGTGTGG |
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ADAMTS-9 |
GGGAGCGGAAACGAAAACCTATT |
167 |
|
CACTGGGCACTACATTCACTCCTG |
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|
ADAMTS-15 |
GACACGGCCATCCTCTTCACTCG |
107 |
|
AGCAGCTCCTCTTGGGGTCACAC |
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|
|
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ADAMTS, a disintegrin and metalloproteinase domain with thrombospondin motifs; α2M, alpha 2 macroglobulin; MMP, matrix metalloproteinase; RECK, reversion-inducing cysteine-rich protein with Kazal motifs; TIMP, tissue inhibitor of metalloproteinase. |
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Milner et al. Arthritis Research & Therapy 2006 8:R142 doi:10.1186/ar2034 |
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