Table 1

Primers for RT-PCR
Gene	Primer	Sequence	RT-PCR product (base pairs)	Annealing temperature	Cycles	Reference

GAPDH	up	CCACCCATGGCAAATTCCATGGCA	600	60	25	Stratagene
	down	TCTAGACGGCAGGTCAGGTCCACC
Actin	up	TGAAGTCTGACGTGGACATC	254	60	25	[7]
	down	ACTCGTCATACTCCTGCTTG
COL1A2	up	AGACCCAAGGACTATGAAGT	509	55	25	[NCBI: NM_000089]
	down	ACATCATTAGAGCCCTGTAG
COL2A1	up	CATCTGGTTTGGAGAAACCATC	606	60	37	[NCBI: J00116]
	down	GCCCAGTTCAGGTCTCTTAG
COL3A1	up	GATGGGGTCAAATGAAGGTGA	546	60	25	[NCBI: NM_000090]
	down	GCAGATGGGCTAGGATTCAAA
COL10A1	up	CCTCTTGTTAGTGCCAACCAG	424	60	37	[NCBI: X98568]
	down	GAGCCACTAGGAATCCTGAG
Aggrecan	up	ACTTCCGCTGGTCAGATGGA	111	55	37	[8]
	down	TCTCGTGCCAGATCATCACC
TGF-β1	up	CAGAAATACAGCAACAATTCCTGG	187	60	37	Stratagene
	down	TTGCAGTGTGTTATCCGTGCTGTC
BMP-7	up	TTTGGGGCCAAGTTTTTCTG	410	60	37	[9]
	down	ACAGGAACTTCCGGGTCAAT
BMP-2	up	GGGAAAACAACCCGGAGATT	503	60	37	[NCBI: NM_001200]
	down	TTAAGGCGTTTCCGCTGTTT
FGF-2	up	TACAACTTCAAGCAGAAGAG	283	60	37	[10]
	down	CAGCTCTTAGCAGACATTGG
VEGF	up	AAGTGGTCCCAGGCTGCA	296	60	37	[NCBI: AY047581]
	down	ATCTCTCCTATGTGCTGGCC
IL-1	up	AAGCAGCCATGGCAGAAGTA	482	60	37	[NCBI: M15840]
	down	GAACACCACTTGTTGCTCCA
TNF-	up	GAGTGACAAGCCTGTAGCCCATGTTGTAGCA	444	60	37	Clontech
	down	GCAATGATCCCAAAGTAGACCTGCCCAGACT

The sources for sequences are given in the last column, the numbers in brackets refer to the literature, and the other numbers indicate the codes from sequences in PubMed. BMP, bone morphogenetic protein; COL, collagen type; FGF, fibroblast growth factor; GAPDH, glucosealdehyde phosphate dehydrogenase; IL, interleukin; RT-PCR, reverse transcription-PCR; TNF, tumor necrosis factor; VEGF, vascular endothelial growth factor.
Neuwirth et al. Arthritis Research & Therapy 2006 8:R92 doi:10.1186/ar1965