Table 1 |
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|
PCR primers |
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| Gene |
Strand |
Sequence |
Annealing temp (°C) |
MgCl2 (mmol/l) |
Product size (bp) |
Reference/GenBank accession number |
|
|
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| Acidic SMase |
Forward |
5'-ATCGGCCTTAATCCTGGTGA-3' |
58 |
2.5 |
151 |
AF325550 |
| Reverse |
5'-GACTGGACACGGAGAGGGC-3' |
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| Neutral SMase |
Forward |
5'-TTGGCAGTGGCCTCTGTGTG-3' |
58 |
2.5 |
151 |
NM031360 |
| Reverse |
5'-AGTCCGCTTAGATGGAGCACC-3' |
|||||
| GAPDH |
Forward |
5'-TGGTCACCAGGGCTGCTTTTA-3' |
60 |
2.5 |
746 |
U85042 |
| Reverse |
5'-CGCCTGCTTCACCACCTTCT-3' |
|||||
| Sox9 |
Forward |
5'-GGGCGAGCCGGACCTGAAGAA-3' |
53 |
1.5 |
321 |
AF278703 |
| Reverse |
5'-CGCTCCGCCTCCTCCACGAAC-3' |
|||||
| Col2a1 |
Forward |
5'-GCCTCGCGGTGAGCCATGATC-3' |
60 |
1.5 |
IIA: 472 |
Valcourt and coworkers [30] |
| Reverse |
5'-CTCCATCTCTGCCACGGGGT-3' |
IIB: 268 |
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|
|
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|
*Unless previously published, primers were designed to GenBank sequences using Primer Express® software (Applied Biosystems). bp, base pairs; SMase, sphingomyelinase. |
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|
Gilbert et al. Arthritis Research & Therapy 2006 8:R89 doi:10.1186/ar1961 |
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