Figure 4.

Human and mouse anti-Su sera immunoprecipitate all four Argonaute (Ago) family members. In vitro translated hAgo1, -2, -3, and -4 were immunoprecipitated using both human and mouse anti-Su sera in comparison with both normal human and pre-immune mouse sera. As a control, the hAgo in vitro transcription and translation mixes were also loaded for migration comparisons.