Table 1 |
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|
Primer sequences used to detect mRNA in mouse costal cartilage explants |
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| Genes |
Temperature (°C) |
Genbank ID |
Forward primer |
Reverse primer |
Amplicon length (bp) |
|
|
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| HPRT |
58 |
NM_013556 |
5'-gctggtgaaaaggacctct-3' |
5'-cacaggactagaacacctgc-3' |
249 |
| COX-1 |
58 |
BC005573 |
5'-ctttgcacaacacttcacccacc-3' |
5'-agcaacccaaacacctcctgg-3' |
285 |
| COX-2 |
58 |
NM_011198 |
5'-gcattctttgcccagcactt-3' |
5'-agaccaggcaccgaccaaaga-3' |
299 |
| COX-3 |
58 |
AY547265 |
5'-tgaacgctaggctcaactctc-3' |
5'-ggttctggcacggatagtaac-3' |
349 |
| cPGES |
58 |
AY281130 |
5'-agtcatggcctaggttaac-3' |
5'-tgtgaatcatcatctgctcc-3' |
196 |
| mPGES-1 |
58 |
NM_022415 |
5'-ctgctggtcatcaagatgtacg-3' |
5'-cccaggtaggccacgtgtgt-3' |
294 |
| mPGES-2 |
58 |
BC004846 |
5'-aagacatgtcccttctgc-3' |
5'-ccaagatgggcactttcc-3' |
133 |
| 15-PGDH |
58 |
NM_008278 |
5'-gccaaggtagcattggtggat-3' |
5'-cttccgaaatggtctacaact-3' |
164 |
|
|
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|
15-PGDH, NAD+-dependent 15 hydroxy prostaglandin dehydrogenase; COX, cyclooxygenase; HPRT, hypoxanthine-guanine phosphoribosyltransferase; cPGES, cytosolic PGES; mPGES, microsomal PGES; PGES, prostaglandin E synthase. |
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|
Gosset et al. Arthritis Research & Therapy 2006 8:R135 doi:10.1186/ar2024 |
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