Figure 2.

Compression stimulates nitric oxide (NO) and prostaglandin E2 (PGE₂) release in mouse costal cartilage explants in the media. Mouse costal cartilage explants were compressed (C) or not (NC) for 2 h, 4 h, 18 h and 24 h. At each time interval, our results are expressed in fold-induction in comparison to the appropriate control. (a) The amount of NO released into the media (μmol/mg of costal cartilage) was measured by Griess reagent. Values are the mean and SEM of 3 (C 2 h and 4 h) and 2 (C 18 h and 24 h) independent experiments with n = 2/group/experiments. ***p < 0.001 versus control (NC). (b) The amount of PGE₂ released into the media (pg/mg/ml of costal cartilage) was measured by enzyme immunoassay. Values are the mean and SEM of 3 (C 2 h), 2 (C 4 h and 18 h) and 4 independent experiments (C 24 h) with n = 2/group/experiments, analyzed in duplicate. ***p < 0.001 versus control (NC).