Research article
A model of anti-angiogenesis: differential transcriptosome profiling of microvascular endothelial cells from diffuse systemic sclerosis patients
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* Corresponding authors: Mario Del Rosso delrosso@unifi.it - Rosanna Abbate r.abbate@dfc.unifi.it
1 Department of Medical and Surgical Critical Care – DENOTHE, University of Florence, Florence, Italy
2 Department of Experimental Pathology and Oncology – DENOTHE, University of Florence, Florence, Italy
3 Department of Internal Medicine, University of Florence, Florence, Italy
4 Division of Rheumatology, Medical College of Ohio, Toledo, Ohio, USA
5 Department of Internal Medicine, University of Pisa, Pisa, Italy
6 Centro S Maria agli Ulivi, Fondazione Don Carlo Gnocchi, ONLUS IRCCS, Impruneta, Florence, Italy
Arthritis Research & Therapy 2006, 8:R115 doi:10.1186/ar2002
Published: 19 July 2006Additional files
Additional File 1:
(a) A PDF file showing the 150 most expressed genes in MVECs, independent of their tissue sample origin (normal subjects, SSc patients). Transcripts are listed according to A* = average of log2 (RxG) of the two arrays, where R and G represent the fluorescence intensity of Cy5 (red) and Cy3 (green). Grouping of various transcripts allows the identification of the following main categories: genes encoding for cytoskeletal elements; genes encoding for proteins that play a role in regulation of actin polymerization; genes regulating detoxification of heavy metals (such as cadmium, zinc and mercury), essential metal homeostasis, protection against radiation and oxidative damage; genes encoding ribosomal proteins; and miscellanea. (b) A PDF file showing the list of all the Gene Ontology (GO) significant terms obtained by the analysis of the 199 differentially expressed genes. This list integrates that shown in Table 1 of the text, where only differentially expressed genes with more than two annotated genes on the array (N > 2) are reported.
Format: PDF Size: 329KB Download file
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Additional File 2:
A PDF file showing the list of differentially expressed genes involved in angiogenesis. This list integrates that shown in Table 2 of the text, starting from transcripts with LOR >0. Transcripts were sub-divided according to their role in various phases of angiogenesis (migration/invasion, proliferation, adhesion, angiogenesis inhibition).
Format: PDF Size: 135KB Download file
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Additional File 3:
A PDF file showing the list of differentially expressed genes involved in apoptosis, haemostasis, inflammation and immunity. This list integrates that shown in Table 2 of the text, starting from transcripts with LOR >0.
Format: PDF Size: 102KB Download file
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Additional File 4:
A PDF file showing the list of differentially expressed genes involved in cellular stress and ubiquitination. This list integrates that shown in Table 2 of the text, starting from transcripts with LOR >0.
Format: PDF Size: 78KB Download file
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Additional File 5:
A PDF file showing the list of differentially expressed genes involved in stimulus transduction, DNA/RNA organization, and transcription. This list integrates that shown in Table 2 of the text, starting from transcripts with LOR >0.
Format: PDF Size: 168KB Download file
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Additional File 6:
A PDF file showing the list of differentially expressed genes involved in regulation of protein synthesis and mitochondrial functions. Genes involved in protein synthesis were identified as structural components of the ribosome or as functional regulators of protein synthesis. All of them were up-regulated in SSc-MVECs, as well as transcripts regulating mitochondrial functions.
Format: PDF Size: 67KB Download file
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Additional File 7:
A PDF file showing the list of differentially expressed genes with unknown function or that cannot be included within a class. Nineteen transcripts were up-regulated and 17 down-regulated in SSc-MVECs.
Format: PDF Size: 97KB Download file
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