Table 1 |
|
|
Probes and primer sequences used |
|
| Gene |
Primers and probes |
|
|
|
| 11β-HSD1 |
Forward primer: AGGAAAGCTCATGGGAGGACTAG |
| Reverse primer: ATGGTGAATATCATCATGAAAAAGATTC |
|
| Probe: CATGCTCATTCTCAACCACATCACCAACA |
|
| H6PDH |
Forward primer: CAGGTGTCCTAGTGCACATTGAC |
| Reverse primer: GTAGCCCACTCTCTCGTCCAA |
|
| Probe: AAGGCACGCCCTCCCAGCG |
|
| GRα |
Forward primer: GCGATGGTCTCAGAAACCAAAC |
| Reverse primer: GAGATTACAGAGGAAGTTATCCTCTGC |
|
| Probe: TGCAGTGAAGGTTGCTGAGGCTCTGA |
|
| GRβ |
Forward primer: AAC TGG CAG CGG TTT TAT CAA CT |
| Reverse primer: AACTCTTGGATTCTATGCATGAAAATGTTA TGTGGTTA |
|
| Probe: TGT GTG AGA TGT GCT TTC TGG TT |
|
| C/EBPα |
Forward primer: TGGACAAGAACAGCAACGAG |
| Reverse primer: TTGTCACTGGTCAGCTCCAG |
|
| Probe: CACCTTCTGCTGCGTCTCCACGTT |
|
| C/EBPβ |
Forward primer: GACAAGCACAGCGACGAGTA |
| Reverse primer: GTGCTGCGTCTCCAGGTT |
|
| Probe: ATCTTGGCCTTGTCGCGGCTCTT |
|
| IL-6 |
Assay on demand Hs00174131_m1 (ABI) |
|
|
|
|
11β-HSD1, 11β-hydroxysteroid dehydrogenase type 1; C/EBP, CCAAT/enhancer binding protein; GR, glucocorticoid receptor; H6PDH, hexose-6-phosphate dehydrogenase. |
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Hardy et al. Arthritis Research & Therapy 2006 8:R108 doi:10.1186/ar1993 |
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