Figure 4.

Site-specific variations in human fibroblastic 11β-HSD1 expression versus other components of glucocorticoid metabolism and signalling. Expression of mRNA for (a) 11β-HSD1, (b) H6PDH, (c) GRα and (d) GRβ in bone marrow (B), and dermal (D) and synovial (S) fibroblasts in the presence or absence of TNF-α (10 ng/ml). For each gene product data were normalized for levels of the housekeeping gene 18S rRNA and are presented as fold change in expression relative to vehicle-treated bone marrow fibroblasts. *P < 0.01 versus vehicle control; ^#P < 0.01 versus equivalent bone marrow fibroblasts; ^&P < 0.01 versus equivalent dermal fibroblasts. 11β-HSD, 11β-hydroxysteroid dehydrogenase type 1; GR, glucocorticoid receptor; H6PDH, hexose-6-phosphate dehydrogenase; TNF, tumour necrosis factor.