Table 1 |
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|
Summary of primers used |
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|
cDNA |
Forward and reverse primers |
Ta (°C) |
Product (bp) |
GenBank |
|
|
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|
IL-1Rrp2 (A) |
F: 5'-AGCAAAATCCCAGTGTCCAAA-3' |
60 |
291 |
[AF284434] |
|
R: 5'-ACCCAAAACACAACTCTTCGG-3' |
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|
IL-1Rrp2 (B) |
F: 5'-AGCAAAATCCCAGTGTCCAAA-3' |
60 |
147 |
[AF284434] |
|
R: 5'-GGTTTACATGTATTCTATGACAG-3' |
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|
IL-1F8 |
F: 5'-ACCAAGGAGAGAGGCATAACTAAT-3' |
60 |
147 |
[NM173178] |
|
R: 5'-AGTGAACTCAGTCGCATAATGATC-3' |
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|
IL-1β |
F: 5'-GCTGAGGAAGATGCTGGTTC-3' |
57 |
146 |
[NM000756] |
|
R: 5'-GTGATCGTACAGGTGCATCG-3' |
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|
β-actin |
F: 5'-CCAAGGCCAACCGCGAGAAGATGAC-3' |
55 |
579 |
[M10277] |
|
R: 5'-AGGGTACATGGTGGTGCCGCCAGAC-3' |
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|
28S |
F: 5'-TTGAAAATCCGCGGGAGA-3' |
54 |
100 |
[U13369] |
|
R: 5'-ACATTGTTCCAACATGCCAG-3' |
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|
|
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|
Shown are the primer sequences, annealing temperatures (Ta), lengths of the corresponding PCR products, and GenBank accession numbers of the DNA sequences. F, forward; IL, interleukin; IL-1Rrp2, IL-1 receptor related protein 2; R, reverse; PCR, polymerase chain reaction. |
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|
Magne et al. Arthritis Research & Therapy 2006 8:R80 doi:10.1186/ar1946 |
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