Table 1

Summary of primers used

cDNA

Forward and reverse primers

Ta (°C)

Product (bp)

GenBank


IL-1Rrp2 (A)

F: 5'-AGCAAAATCCCAGTGTCCAAA-3'

60

291

[AF284434]

R: 5'-ACCCAAAACACAACTCTTCGG-3'

IL-1Rrp2 (B)

F: 5'-AGCAAAATCCCAGTGTCCAAA-3'

60

147

[AF284434]

R: 5'-GGTTTACATGTATTCTATGACAG-3'

IL-1F8

F: 5'-ACCAAGGAGAGAGGCATAACTAAT-3'

60

147

[NM173178]

R: 5'-AGTGAACTCAGTCGCATAATGATC-3'

IL-1β

F: 5'-GCTGAGGAAGATGCTGGTTC-3'

57

146

[NM000756]

R: 5'-GTGATCGTACAGGTGCATCG-3'

β-actin

F: 5'-CCAAGGCCAACCGCGAGAAGATGAC-3'

55

579

[M10277]

R: 5'-AGGGTACATGGTGGTGCCGCCAGAC-3'

28S

F: 5'-TTGAAAATCCGCGGGAGA-3'

54

100

[U13369]

R: 5'-ACATTGTTCCAACATGCCAG-3'


Shown are the primer sequences, annealing temperatures (Ta), lengths of the corresponding PCR products, and GenBank accession numbers of the DNA sequences. F, forward; IL, interleukin; IL-1Rrp2, IL-1 receptor related protein 2; R, reverse; PCR, polymerase chain reaction.

Magne et al. Arthritis Research & Therapy 2006 8:R80   doi:10.1186/ar1946

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