Research article
CD95-induced osteoarthritic chondrocyte apoptosis and necrosis: dependency on p38 mitogen-activated protein kinase
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* Corresponding author: Lei Wei lwei@lifespan.org
Department of Orthopaedics, Brown Medical School/Rhode Island Hospital, Providence, Rhode Island, USA
Arthritis Research & Therapy 2006, 8:R37 doi:10.1186/ar1891
Published: 16 January 2006Additional files
Additional File 1:
A figure showing anti-CD95 stimulated CD95L mRNA levels, as identified using the SYBR green real-time RT-PCR method. Briefly, 1 μg total RNA was reverse-transcribed into cDNA using iScripTM (Bio-Rad). Real-time quantitative PCR amplification was performed using QuantiTect SYBR Green PCR kit (Qiagen, Valencia, CA, USA) with DNA Engine Opticon 2 Continuous Fluorescence Detection System (MJ Research, Waltham, MA, USA).
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Additional File 2:
A figure showing no significant difference in the phosphorylation of p38, ATF-2, c-Jun amino-terminal kinase (JNK), and extracellular signal-regulated kinase (ERK) between chondrocytes treated with or without an IgM isotype control antibody.
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Additional File 3:
A figure showing that serum starvation will not effect cell death in an incubation period of 24 hours. Cell death was detected by trypan blue incorporation.
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