Table 1

Primer pairs used to amplify specific FCGR sequencing templates

Gene

Forward primer

Sequence

Reverse primer

Sequence

Annealing temperature (°C)


Sequencing primers

FCGR2A

IIA-SF

dGGAGAAACCATCATGCTGAG

IIA-SR

dCAATTTTGCTGCTATGGGC

52

FCGR2B (E3)

IIB-E3SF

dGGCATCTCAAGCTCC

IIB-E3SR

dAGAGTCACAGAGTCCTCC

58

FCGR2B (E6)

IIB-E6SF

dCCCATCCAACCCTGG

IIB-E6SR

dGGCAGATTCCTCAGCAAATCA

56

FCGR2B (3'UTR)

IIB-UTRSF

dTGGGGAGGACAGGGAGAT

IIB-UTRSR

dATCACTTTTAATGTGCTGGTAGAGG

63

Genotyping primers

FCGR2A

PCR1

dGGAGAAACCATCATGCTGAG

4INM

dCAATTTTGCTGCTATGGGC

52

IIA-R

dAATCCCAGAAATTCTCCCG

IIA-H

dAATCCCAGAAATTCTCCCA

FCGR3B-NA1

IIIB-NA1F

dCAGTGGTTTCACAATGTGAA

IIIB-NA1R

dATGGACTTCTAGCTGCACCG

54

IIIB-NA1PR

dGTCTCTTTCTGCTTGGTGATGG

IIIB-NA1PR

dTTTTCCCCTCTAAACTGGG

FCGR3B-NA2

IIIB-NA2F

dCTCAATGGTACAGCGTGCTT

IIIB-NA2R

dCTGTACTCTCCACTGTCGTT

62

IIIB-NA2PR

dCTGGCTTGCTGATGAAGATAC

IIIB-NA2PR

dGTAACGCTTNGGCACCACC

FCGR2B

IIB-UTRSF

dTGGGGAGGACAGGGAGAT

IIB-UTRGR

dCAGAAGGTGCAGTCGGC

50


The mutation inserted into the FCGR2B reverse nested primer (IIB-UTRGR) is shown in italics. This introduced an allele-specific HaeIII restriction site (GGCC) in the presence of the G but not the A allele.

Morgan et al. Arthritis Research & Therapy 2006 8:R5   doi:10.1186/ar1847

Open Data