Table 1

*Primer pairs used to amplify specific FCGR* sequencing templates**
Gene	Forward primer	Sequence	Reverse primer	Sequence	Annealing temperature (°C)

Sequencing primers
FCGR2A	IIA-SF	dGGAGAAACCATCATGCTGAG	IIA-SR	dCAATTTTGCTGCTATGGGC	52
FCGR2B (E3)	IIB-E3SF	dGGCATCTCAAGCTCC	IIB-E3SR	dAGAGTCACAGAGTCCTCC	58
FCGR2B (E6)	IIB-E6SF	dCCCATCCAACCCTGG	IIB-E6SR	dGGCAGATTCCTCAGCAAATCA	56
FCGR2B (3'UTR)	IIB-UTRSF	dTGGGGAGGACAGGGAGAT	IIB-UTRSR	dATCACTTTTAATGTGCTGGTAGAGG	63
Genotyping primers
FCGR2A	PCR1	dGGAGAAACCATCATGCTGAG	4INM	dCAATTTTGCTGCTATGGGC	52
	IIA-R	dAATCCCAGAAATTCTCCCG	IIA-H	dAATCCCAGAAATTCTCCCA
FCGR3B-NA1	IIIB-NA1F	dCAGTGGTTTCACAATGTGAA	IIIB-NA1R	dATGGACTTCTAGCTGCACCG	54
	IIIB-NA1PR	dGTCTCTTTCTGCTTGGTGATGG	IIIB-NA1PR	dTTTTCCCCTCTAAACTGGG
FCGR3B-NA2	IIIB-NA2F	dCTCAATGGTACAGCGTGCTT	IIIB-NA2R	dCTGTACTCTCCACTGTCGTT	62
	IIIB-NA2PR	dCTGGCTTGCTGATGAAGATAC	IIIB-NA2PR	dGTAACGCTTNGGCACCACC
FCGR2B	IIB-UTRSF	dTGGGGAGGACAGGGAGAT	IIB-UTRGR	dCAGAAGGTGCAGTCGGC	50

The mutation inserted into the FCGR2B reverse nested primer (IIB-UTRGR) is shown in italics. This introduced an allele-specific HaeIII restriction site (GGCC) in the presence of the G but not the A allele.
Morgan et al. Arthritis Research & Therapy 2006 8:R5 doi:10.1186/ar1847