Table 1 |
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|
Primer pairs used to amplify specific FCGR sequencing templates |
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|
Gene |
Forward primer |
Sequence |
Reverse primer |
Sequence |
Annealing temperature (°C) |
|
|
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|
Sequencing primers |
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|
FCGR2A |
IIA-SF |
dGGAGAAACCATCATGCTGAG |
IIA-SR |
dCAATTTTGCTGCTATGGGC |
52 |
|
FCGR2B (E3) |
IIB-E3SF |
dGGCATCTCAAGCTCC |
IIB-E3SR |
dAGAGTCACAGAGTCCTCC |
58 |
|
FCGR2B (E6) |
IIB-E6SF |
dCCCATCCAACCCTGG |
IIB-E6SR |
dGGCAGATTCCTCAGCAAATCA |
56 |
|
FCGR2B (3'UTR) |
IIB-UTRSF |
dTGGGGAGGACAGGGAGAT |
IIB-UTRSR |
dATCACTTTTAATGTGCTGGTAGAGG |
63 |
|
Genotyping primers |
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|
FCGR2A |
PCR1 |
dGGAGAAACCATCATGCTGAG |
4INM |
dCAATTTTGCTGCTATGGGC |
52 |
|
IIA-R |
dAATCCCAGAAATTCTCCCG |
IIA-H |
dAATCCCAGAAATTCTCCCA |
||
|
FCGR3B-NA1 |
IIIB-NA1F |
dCAGTGGTTTCACAATGTGAA |
IIIB-NA1R |
dATGGACTTCTAGCTGCACCG |
54 |
|
IIIB-NA1PR |
dGTCTCTTTCTGCTTGGTGATGG |
IIIB-NA1PR |
dTTTTCCCCTCTAAACTGGG |
||
|
FCGR3B-NA2 |
IIIB-NA2F |
dCTCAATGGTACAGCGTGCTT |
IIIB-NA2R |
dCTGTACTCTCCACTGTCGTT |
62 |
|
IIIB-NA2PR |
dCTGGCTTGCTGATGAAGATAC |
IIIB-NA2PR |
dGTAACGCTTNGGCACCACC |
||
|
FCGR2B |
IIB-UTRSF |
dTGGGGAGGACAGGGAGAT |
IIB-UTRGR |
dCAGAAGGTGCAGTCGGC |
50 |
|
|
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|
The mutation inserted into the FCGR2B reverse nested primer (IIB-UTRGR) is shown in italics. This introduced an allele-specific HaeIII restriction site (GGCC) in the presence of the G but not the A allele. |
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|
Morgan et al. Arthritis Research & Therapy 2006 8:R5 doi:10.1186/ar1847 |
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