Table 3

Reactivity in ELISA of sera from mothers of groups I and II
Antigen
 Systemic lupus erythematosus
 Sjögren's syndrome
 Asymptomatic


Group I (%)
 Group II (%)
 P
 Group I (%)
 Group II (%)
 P
 Group I (%)
Recombinant Ro52 protein
 97
 66
 0.009*
 67
 98
 0.027*
 31
1–13 MASAARLTMMWEE
 77
 46
 0.084
 47
 58
31
10–25 MWEEVTPICLDPFVE
 0
 14
0
 0
0
12–35 EEVTCPICLDPFVEPVSIECGHSF
 10
 16
20
 8
13
25–40 EPVSIECGHSFCQECI
 0
 13
10
 0
6
34–56 SFCQECISQVGKGGGSVCAVCRQ
 0
 1
7
 0
13
38–53 ECISQVGKGGGSVCAV
 3
 4
7
 0
0
50–64 VCAVCRQRFLLKNLR
 25
 3
13
 5
6
63–79 LRPNRQLANMVNNLKEI
 0
 6
0
 3
0
77–94 KEISQEAREGTQGERCAV
 18
 1
3
 0
0
93–116 AVHGERLHLFCEKDGKALCWVCAQ
 40
 12
10
 9
0
94–109 VHGERLHLFCEKDGKA
 3
 13
0
 0
0
107–122 GKALCWVCAQSRKHRD
 69
 28
 0.023*
 47
 33
13
107–126 GKALCWVCAQSRKHRDHAMV
 37
 7
 0.062
 13
 8
13
121–137 RDHAMVPLEEAAQEYQE
 0
 4
0
 0
0
135–151 YQEKLQVALGELRRKQE
 3
 14
0
 0
0
149–166 KQELAEKLEVEIAIKRAD
 0
 12
0
 1
0
164–180 RADWKKTVETQKSRIHA
 0
 9
0
 0
6
178–193 IHAEFVQQKNFLVEEE
 3
 14
0
 0
0
191–208 EEEQRQLQELEKDEREQL
 0
 0
0
 0
0
206–224 EQLRILGEKEAKLAQQSQA
 0
 1
7
 0
9
222–235 SQALQELISELDRRCHS
 17
 3
23
 1
6
236–250 CHSSALELLQEVIIV
 27
 6
10
 7
0
249–264 IVLERSESWNLKDLDI
 0
 5
7
 0
0
262–279 LDITSPELRSVCHVPGLK
 0
 0
0
 0
0
277–292 GLKKMLRTCAVHITLD
 83
 49
 0.049*
 40
 24
6
290–304 TLDPDTANPWLILSE
 0
 7
7
 0
0
302–317 LSEDRRQVRLGDTQQS
 10
 2
7
 0
0
314–328 TQQSIPGNEERFDSY
 0
 9
0
 9
0
326–340 DSYPMVLGAQHFHSG
 0
 0
0
 0
0
337–352 FHSGKHYWEVDVTGKE
 23
 11
3
 12
6
350–367 GKEAWDLGVCRDSVRRKG
 0
 1
0
 0
9
365–382 RKGHFLLSSKSGFWTIWL
 77
 47
 0.054
 50
 33
25
380–396 IWLWNKQKYEAGTYPQT
 0
 5
0
 2
0
394–412 PQTPLHLQVPPCQVGIFLD
 18
 1
3
 10
0
411–423 LDYEAGMVSFYNI
 0
 2
0
 0
0
422–438 NITDHGSLIYSFSECAF
 16
 2
0
 0
0
436–451 CAFTGPLRPFFSPGFN
 13
 7
13
 10
0
447–463 SPGFNDGGKNTAPLTLC
 8
 9
0
 6
0
463–475 CPLNIGSQGSTDY
 0
 7
7
 1
0
Ro60 PROTEIN
 80
 67
67
 99
 0.022*
 31
1–23 MEESVNQMQPLNEKQIANSQDGY
 15
 6
17
 1
6
18–38 NSQDGYVWQVTDMNRLHRFLC
 32
 11
20
 1
 0.097
 0
21–41 DGYVWQVTDMNRLHRFLCFGS
 64
 27
 0.044*
 23
 38
0
304–324 VCEKLCNEKLLKKARIHPFHI
 50
 18
27
 4
13
495–518 KLIVCGMTSNGFTIADPDDRGMLD
 10
 24
 0.058
 7
 11
 0.079
 13
524–538 TGALDVIRNFTLDMI
 10
 17
3
 9
0
La PROTEIN
 93
 30
 <0.0001*
 40
 68
13

The 192 samples described in Table 1 were systematically tested in at least two independent tests. Only the IgG response was tested. Patients' sera were diluted 1:1,000. To be tested in the ELISA, peptide 21–41 of Ro60 protein was conjugated to ovalbumin. The sera were considered positive when optical density ≥0.3. Statistically significant differences between groups were determined by Student's t test. *P < 0.05 considered significant.

Fritsch et al. Arthritis Research & Therapy 2006 8:R4   doi:10.1186/ar1848

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