Representative Western blot of time course of MMP-13-induced degradation of biglycan. Human articular cartilage extracts were incubated with APMA-activated MMP-13 for the indicated times (0–16 hours). Panels are for extracts from (a) normal (nonfibrillated) cartilage and from (b) slightly, (c) moderately and (d) severely fibrillated OA cartilage. The bottom panel (e) relates to the extract from moderately fibrillated OA cartilage incubated for 1.5 hours with APMA-activated MMP-13 in the absence or presence of 50 or 1 nmol/l RS 110–2481 (a preferential MMP-13 inhibitor). APMA, aminophenylmercuric acetate; MMP, matrix metalloprotease; OA, osteoarthritis; rh, human recombinant.
Monfort et al. Arthritis Research & Therapy 2006 8:R26 doi:10.1186/ar1873