Research article
Partial protection against collagen antibody-induced arthritis in PARP-1 deficient mice
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* Corresponding author: Carmen Conde Carmen.Conde.Muro@sergas.es
1 Research Laboratory, Hospital Clínico Universitario, Choupana s/n, 15706-Santiago de Compostela, Spain
2 Department of Pathology, Hospital Clínico Universitario, Choupana s/n, 15706-Santiago de Compostela, Spain
3 Rheumatology Unit, Hospital Clínico Universitario and Department of Medicine, Universidad de Santiago, San Francisco s/n, 15700-Santiago de Compostela, Spain
Arthritis Research & Therapy 2006, 8:R14 doi:10.1186/ar1865
Published: 6 December 2005Abstract
Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear DNA-binding protein that participates in the regulation of DNA repair and maintenance of genomic integrity. In addition, PARP-1 has a role in several models of inflammation disease, where its absence or inactivation confers protection. The aim of this study was to analyze the impact of selective PARP-1 suppression in collagen antibody-induced arthritis. We show that PARP-1 deficiency partially reduces the severity of arthritis, although the incidence of disease was similar in control and deficient mice. Decreased clinical scores were accompanied by partial reduction of histopathological findings. Interestingly, quantitative real-time PCR and ELISA analysis revealed that the absence of PARP-1 down-regulated IL-1β and monocyte chemotactic protein 1 expression in arthritic joints whereas tumor necrosis factor-α transcription was not impaired. Our results provide evidence of the contribution of PARP-1 to the progression of arthritis and identify this protein as a potential therapeutic target for the treatment of rheumatoid arthritis.