Figure 7.

Comparison of ELISAs performed with mPEGs and PEG-uricase. ELISA plates were coated with 2.5 μg of PEG-modified recombinant mammalian urate oxidase (PEG-uricase) or with 25 μg of p-nitrophenyl carbonate-activated monomethoxyPEGs (mPEGs) of molecular mass 5 kDa (mPEG-5K) or 10 kDa (mPEG-10K). After washing, the plates were blocked with 1% BSA, 1% glycine in PBS. The indicated plasma samples obtained on day 0 or day 21 after subcutaneous injection of PEG-uricase were then assayed with each substrate at a dilution of 1:50 as described in the Materials and methods section for the PEG-uricase ELISA.