Table 1 |
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|
Sequences of the primers used for RT-PCR |
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|
Primer's name |
Product |
Oligonucleotide sequence |
Size (base pairs) |
Accession number |
|
|
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|
ADAM8 |
Forward |
5'-GCCGTCTTCAGGCCTCGGCCCGGGGACTCT-3' |
651 |
NM001109 |
|
Reverse |
5'-AGGGGCGTTGGCGAGGCACACCGACTGCGG-3' |
|||
|
ADAM9 |
Forward |
5'-GCTGTCTTGCCACAGACCCGGTATGTGGAG-3' |
604 |
HSU41766 |
|
Reverse |
5'-TGGAATATTAAGAAGGCAGTTTCCTCCTTT-3' |
|||
|
ADAM10 |
Forward |
5'-ATCCAGTCATGTTAAAGCGATTGATACAATTTAC-3' |
434 |
NM001110 |
|
Reverse |
5'-TCCAAAGTTATGTCCAACTTCGTGAGCAAAAGTAA-3' |
|||
|
ADAM12 |
Forward |
5'-GAGACCCTCAAGGCAACTAAGTATGTGGAG-3' |
627 |
AF023476 |
|
Reverse |
5'-CGGCAGGTTAAACAGGCACACCCCCATTCC-3' |
|||
|
ADAM15 |
Forward |
5'-CTGGGACAGCGCCACATTCGCCGGAGGCGG-3' |
688 |
HSU41767 |
|
Reverse |
5'-TCCGCAGAAAGCAGCCATAGGGGGTAGGCT-3' |
|||
|
ADAM17 |
Forward |
5'-AGAGCTGACCCAGATCCCATGAAGAACACG-3' |
777 |
HSU69611 |
|
Reverse |
5'-GCGTTCTTGAAAACACTCCTGGGCCTTACT-3' |
|||
|
ADAM20 |
Forward |
5'-AAAATAGCACACCAGATGGAGTTGCAATTG-3' |
702 |
AF029899 |
|
Reverse |
5'-ATTCCCACAGTACTTCAGTCTAAATATATT-3' |
|||
|
ADAM21 |
Forward |
5'-TCTGGCTTGGGGTATTTTTG-3' |
500 |
AF158644 |
|
Reverse |
5'-TTGGCGTGCTACTTCCTTCT-3' |
|||
|
ADAM28 |
Forward |
5'-GCTGTGATGCTAAGACATGT-3' |
871 |
AF137334 |
|
Reverse |
5'-TGAACAGCCTTTACCATCTG-3' |
|||
|
ADAM30 |
Forward |
5'-AACCAGGTGCCAACTGTAGC-3' |
496 |
AF171932 |
|
Reverse |
5'-CCCATGGGTTTCATGGATAG-3' |
|||
|
VEGFR-1 |
Forward |
5'-GATGTTGAGGAAGAGGAGGATT-3' |
1146 |
NM002019 |
|
Reverse |
5'-AAGCTAGTTTCCTGGGGGTATA-3' |
|||
|
VEGFR-2 |
Forward |
5'-GATGTGGTTCTGAGTCCGTCT-3' |
562 |
NM002253 |
|
Reverse |
5'-CATGGCTCTGCTTCTCCTTTG-3' |
|||
|
Neuropilin-1 |
Forward |
5'-CAACGATAAATGTGGCGATACT-3' |
824 |
NM003873 |
|
Reverse |
5'-TATACTGGGAAGAAGCTGTGAT-3' |
|||
|
CD31 |
Forward |
5'-CAACGAGAAAATGTCAGA-3' |
259 |
NM000442 |
|
Reverse |
5'-GGAGCCTTCCGTTCTAGAGT-3' |
|||
|
vWF |
Forward |
5'-GTTCGTCCTGGAAGGATCGG-3' |
697 |
NM000552 |
|
Reverse |
5'-CACTGACACCTGAGTGAGAC-3' |
|||
|
β-Actin |
Forward |
5'-TGACGGGGTCACCCACACTGTGCCCATCTA-3' |
661 |
NM001101 |
|
Reverse |
5'-CTAGAAGCATTTGCGGTGGACGATGGAGGG-3' |
|||
|
|
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|
ADAM, a disintegrin and metalloproteinase; VEGFR, vascular endothelial growth factor receptor; vWF, von Willebrand factor. |
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|
Komiya et al. Arthritis Research & Therapy 2005 7:R1158 doi:10.1186/ar1796 |
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