Table 1 |
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Epitope sequences and epitope specificities of mAbs |
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|
Epitope |
Epitope sequence (epitope in THP)a |
MAbs |
Antigen reactivityb |
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|
|
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|
Clone |
Isotype |
CII |
C1 |
J1 |
T1 |
U1 |
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|
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|
C1 |
GPBGPBGPBGPBGPBG-ARGLTGRBGDA-GPBGPBG-εACA |
CIIC1 |
IgG2a |
1.7 |
2.5 |
0.0 |
0.0 |
0.0 |
|
J1 |
GPBGPBGPBGPBGPBG-MBGERGAAGIAGPK-GPBGPBG-εACA |
M2139 |
IgG2b |
2.4 |
0.0 |
2.5 |
0.0 |
0.0 |
|
T1 |
GPBGPBGPBGPBGPBG-IAGFKGEQGPKGEP-GPBGPBG-εACA |
122.9 |
IgG2a |
1.7 |
0.0 |
0.0 |
2.5 |
0.0 |
|
U1 |
GPBGPBGPBGPBGPBG-LVGPRGERGFB-GPBGPBG-εACA |
UL1 |
IgG2b |
2.1 |
0.0 |
0.0 |
0.0 |
2.4 |
|
|
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aAll epitopes were in native triple-helical form. Amino acids are abbreviated as follows: G, glycine; P, proline; B, hydroxyproline; E, glutamic acid; R, arginine; K, lysine; H, histidine; F, phenylalanine; A, alanine; L, leucine; T, threonine; Y, tyrosine; εACA, ε-aminohexanoic acid-lysine-lysine-tyrosine-glycine-OH. bAbsorbance at 405 nm in ELISA tested with affinity-purified mAbs. In the ELISA plates, all the triple-helical peptides were coated at 4 μg/ml, whereas type II collagen (CII) was coated at 10 μg/ml. CIIC1, M2139 and UL1 are mouse monoclonal antibodies and were all tested at a concentration of 1 μg/ml; 122.9 is a rat monoclonal antibody and was tested with culture supernatant. |
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Bajtner et al. Arthritis Research & Therapy 2005 7:R1148 doi:10.1186/ar1800 |
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