Figure 1.

Western blot analysis of autoantibodies in selected BD sera. Whole cell extracts from bovine aortic endothelial cells were separated by a 7.5% gel SDS-PAGE, transferred to nitrocellulose and probed with serum dilutions of 1:100. Several sera showed reactivity to proteins of 49, 120 and 150 kDa. The serum in lane 2 with the strongest reactivity to the 49 kDa and 120 kDa bands was selected as the probe for the expression library screening. Molecular mass markers are shown on the left. NHS represents the normal control. BD, Behçet's disease.