Figure 2.

Proliferating cell nuclear antigen (PCNA) expression in cultured human THP-1 cell line. (a) Proliferating cell nuclear antigen (PCNA) expression in the cultured human THP-1 cell line after hormonal treatment. Results are expressed as the mean ± standard deviation of the percentage of staining area (positive area defined as the number of pixels detectable per micron square) for 100 cells in five different experiments. Bottom: western blot analysis. (b) PCNA expression in untreated THP-1 cells (control [cnt]). (c) PCNA expression in THP-1 cells treated with testosterone (T) after 168 hours. (d) PCNA expression in THP-1 cells treated with 17β-oestradiol (E2) after 168 hours (magnification, × 500).