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PCR primers and sequence start position for amplification of MT-ND1 and DLD |
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| Primer name |
Start position |
Sequence 5'-3' |
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| mtMT-ND1a |
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| F1A |
2995 |
TTGGATCAGGACATCCCGA |
| R1A |
3645 |
ACGGCTAGGCTAGAGGTGG |
| F1B |
3536 |
TTAGCTCTCACCATCGCT |
| R1B |
4239 |
ATTGTAATGGGTATGGAGACA |
| F2 |
4184 |
TTCCTACCACTCACCCTAG |
| R2 |
4869 |
CATGTGAGAAGAAGCAG |
| DLDb |
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| DLD – sense |
417 |
ATGATGGAGCAGAAGAGTACTGCA |
| DLD – antisense |
1088 |
TTTAGTTTGAAATCTGGTATTGAC |
|
aSee Fig. 1 for position of primers. Both forward and reverse primers in addition to the specific nucleotide sequence have a corresponding M13 tag (M13F, 5'-TGTAAAACGACGGCCAGT- 3' ; M13R, 5'-CAGGAAACAGCTATGACC-3'); start position numbering represents location of 5' end corresponding to the Anderson Reference Sequence [17]. bStart position numbering represents location of the 5' end and corresponds to the DLD cDNA numbering system published by Pons and colleagues [19]. mt, mitochondrial. | ||
Da Sylva et al. Arthritis Research & Therapy 2005 7:R844 doi:10.1186/ar1752 |
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