Research article
Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens
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* Corresponding author: Masato Nose masanose@m.ehime-u.ac.jp
1 Ehime University School of Medicine, Ehime, Japan
2 Otsuka Pharmaceutical Co Ltd, Tokushima, Japan
3 Center for Rheumatic Diseases, Matsuyama Red Cross Hospital, Ehime, Japan
Arthritis Research & Therapy 2005, 7:R825-R836 doi:10.1186/ar1751
Published: 25 April 2005Additional files
Additional File 1:
A PDF showing a dendrogram of two-dimensional hierarchical clustering analysis of 1,035 genes among 12 patients with early rheumatoid arthritis (RA), not including the cases with long-standing RA. On the horizontal axis, 12 samples from early RA are clustered into two major groups. The results were similar to those shown in Fig. 3. This may indicate that there was no influence of the cases with long-standing RA in the cluster analysis.
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Additional File 2:
A PDF file showing the results of RT-PCR of multilayered lining tissues. (A) Signal intensity of candidate genes in microarray of the four cases of early RA; (B) their RT-PCR results. The expression levels of these genes themselves seemed to be well correlated in the two assays.
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Additional File 3:
A PDF file showing dendrograms of two-dimensional hierarchical clustering analysis with two different similarity measures and with two kinds of cutoff value for signal intensities among 18 samples from the 16 cases of rheumatoid synovitis. (Similarity measures: Euclidean distance and Pearson correlation coefficient. Cutoff value for signal intensities: 10,000 and 20,000.) There was no major difference between them regarding the cases belonging to each group.
Format: PDF Size: 1.8MB Download file
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