Table 1

Results of the technical comparison of indirect immunofluorescence (IIF), immunoblot and ELISA for the detection of anti-polymyositis/scleroderma (anti-PM/Scl) antibodies

Sample number

IIF

Immunoblot

ELISA


Titer

Pattern

PM/Scl-75

PM/Scl-100

Other

PM/Scl-100

PM1-α


1

1:10000

FG, N

++

+++

15.3

17.7

2

1:1000

FG, N

+

+

2.9

7.6

3

1:3200

FG, N

++

++

14.4

29.2

1:320

AMA

4

1:320

FG, N

-

+

1.1

4.7

5

1:1000

FG, N

+

+

2.2

12.1

6

1:3200

FG, N

-

-

0.6

2.3

7

1:1000

FG, N

++

+++

5.2

13.3

8

1:1000

FG, N

+

+

2.3

12.7

9

1:3200

FG, N

+

+++

P38

15.5

36.1

10

-

-

+

1.7

4.0

11

1:1000

FG, N

-

++

19.2

2.4

12

1:1000

FG, N

++

+

1.2

3.2

13

1:3200

FG, N

+++

+++

Ku86, Jo-1

6.5

10.6

14

1:3200

FG, N

-

++

2.1

5.9

15

1:3200

FG, N

++

+++

11.4

22.5

16

1:1000

FG, N

-

+

3.6

8.5

17

1:1000

FG, N

+

++

3.2

15.0

18

1:3200

FG, N

++

++

6.1

11.8

19

n.d.

n.d.

-

++

7.1

19.4

20

1:320

N

-

+

2.1

5.6

21

n.d.

n.d.

+++

++

4.1

12.8

22

1:10000

FG

-

+

P38

0.7

1.5

1:10000

SPA

23

1:1000

N

n.d.

n.d.

0.5

2.7

24

n.d.

n.d.

++

+++

18.2

34.7

25

1:10000

N

n.d.

n.d.

P38

0.5

2.0

1:1000

Rib

26

1:1000

N

n.d.

n.d.

1.9

8.4

27

n.d.

n.d.

+

++

5.9

19.4

28

n.d.

n.d.

++

+++

20.9

37.3

29

1:1000

Hom

n.d.

n.d.

Ku86, Cen, M2

0.6

0.8

1:100

N*

30

1:1000

N

-

+

0.6

1.4

31

1:3200

Hom, N

-

-

0.5

2.9

32

1:3200

N

-

-

0.5

1.3

33

1:320

FG, N

+

++

7.2

25.7

Number positive/tested

26/29

17/29

27/29

26/33

32/33


-, negative; +, weak positive; ++, positive; +++, strong positive; n.d., not determined; FG, fine granular; Hom, homogenous; SPA, spindle apparatus; N, nucleoli; AMA, anti-mitochondrial antibodies; Cen, centromere; Rib, ribosomal. * Primate liver.

Mahler et al. Arthritis Research & Therapy 2005 7:R704-R713   doi:10.1186/ar1729

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