Figure 6.

LEF-M suppresses LPS-induced NF-κB activation in DCs. Immature dendritic cells (DCs) were cultured for 2 hours with or without the active metabolite of leflunomide (LEF-M; 150 μmol/l), followed by addition of lipopolysaccharide (LPS; 100 ng/ml) or medium as control. After 40 and 70 min total nucleoprotein was extracted and nuclear factor-κB (NF-κB) activity was detected using electrophoretic mobility shoft assay. Similar results were obtained in two independent experiments. (Nonspecific bands are labelled NS.)