Table 1

Anti-CD134 5'-fluoro-2'-deoxyuridine dipalmitate liposomes inhibit the proliferation of CD134+ T cells in vitro

Drug

Drug carrier

Targeting moiety

Inhibition of proliferation (%)


None

None

None

0.0 ± 8.2

FUdR

None

Anti-CD134

8.9 ± 8.7

FUdR-dP

Liposomes

None

6.3 ± 0.7

None

Liposomes

Anti-CD134

0.6 ± 1.1

FUdR-dP

Liposomes

Anti-CD134

31.0 ± 2.3*


Con A-activated CD134+ T cells of clone A2b were incubated for 30 min without ('none' under the heading 'drug') or with either free 5'-fluoro-2'-deoxyuridine (FUdR), 5'-fluoro-2'-deoxyuridine dipalmitate (FUdR-dP) liposomes, anti-CD134 liposomes, or anti-CD134-FUdR-dP liposomes. To each well was added 100 nM FUdR or 1 nmol of liposomal lipid (for FUdR-dP liposomes this equals 100 nM FUdR). Subsequently, cells were washed and cultured for 48 hours, followed by [3H]thymidine incorporation as a measure of proliferation. Results are expressed as the mean percentage of inhibition of proliferation relative to the incubation without liposomes (defined as 0%; mean [3H]thymidine incorporation of 53,330 c.p.m.). Results in the last column are means ± SEM. One representative experiment of three is shown. *P < 0.001 compared with incubation without liposomes.

Boot et al. Arthritis Research & Therapy 2005 7:R604-R615   doi:10.1186/ar1722

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