Leptin acts synergistically with IL-1 in human primary chondrocytes. Nitrite (NO2-) accumulation in leptin (OB)/IL-1 co-stimulated human primary chondrocytes. Stimulations were conducted in serum-free conditions in the presence or absence of tyrphostin AG490, wortmannin, LY294002, PD98059 and SB203580 inhibitors. Values are expressed as mean ± standard error of the mean.
Otero et al. Arthritis Research & Therapy 2005 7:R581 doi:10.1186/ar1708