Histology of cell-seeded scaffolds. The figure shows scaffolds seeded with chondrocytes from one representative autologous chondrocyte transplantation (ACT) donor (a–c) and one osteoarthritis (OA) donor (unaffected) at two different cell seeding densities, 106 cells/cm2 (d–f) and 5 × 106 cells/cm2 (g–i). Accumulation of proteoglycans is shown with Alcian blue/van Gieson stain and the presence of type II collagen is indicated by red staining within the scaffold. Panels (b), (e) and (h) are higher magnifications of portions of (a), (d) and (g), respectively.
Tallheden et al. Arthritis Research & Therapy 2005 7:R560 doi:10.1186/ar1709