Table 3 |
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|
Effect of synovial microparticles on the release of inflammatory mediators by fibroblast-like synoviocytes from arthritic patients (n = 11) |
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|
Mediator |
Control |
P* |
MP-free synovial fluid |
MP (1×) |
Nx/Nt |
P† |
MP (3×) |
Nx/Nt |
P‡ |
|
|
|
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|
Unstimulated |
IL-1β |
|||||||||
|
|
||||||||||
|
MCP-1 (pg/ml) |
456 |
4,754 |
0.001 |
469 |
488 |
6/11 |
0.010 |
900 |
4/6 |
0.156 |
|
(355–1,292) |
(2,492–6,081) |
(293–1,241) |
(338–1,481) |
higher |
(346–2,326) |
higher |
||||
|
sICAM-1 (ng/ml) |
0.09 |
0.40 |
0.007 |
1.04 |
2.00 |
8/11 |
0.010 |
6.07 |
6/6 |
0.031 |
|
(0–0.3) |
(0–0.76) |
(0.34–1.84) |
(0.35–4.07) |
higher |
(0.91–11.75) |
higher |
||||
|
IL-8 (pg/ml) |
0 |
8,642 |
0.001 |
26 |
301 |
5/11 |
0.008 |
790 |
6/6 |
0.031 |
|
(0–564) |
(2,954–18,330) |
(0–528) |
(0–707) |
higher |
(0–2,100) |
higher |
||||
|
IL-6 (pg/ml) |
74 |
4949 |
0.001 |
110 |
136 |
7/11 |
0.042 |
436 |
6/6 |
0.031 |
|
(24–1,710) |
(1,870–22,797) |
(30–1,176) |
(34–1,937) |
higher |
(44–3,766) |
higher |
||||
|
VEGF (pg/ml) |
48 |
79 |
0.014 |
34 |
74 |
10/11 |
0.001 |
111 |
6/6 |
0.078 |
|
(11–102) |
(7–141) |
(1–97) |
(28–138) |
higher |
(27–161) |
higher |
||||
|
GM-CSF (pg/ml) |
32 |
53 |
0.004 |
31 |
22 |
10/11 |
0.002 |
18 |
6/6 |
0.016 |
|
(28–40) |
(40–72) |
(26–70) |
(14–43) |
lower |
(14–25) |
lower |
||||
|
RANTES (pg/ml) |
0 |
138 |
0.001 |
0 |
0.2 |
4/11 |
0.031 |
4.2 |
5/6 |
0.062 |
|
(0–74) |
(46–277) |
(0–58) |
(0–86) |
higher |
(0–32) |
higher |
||||
|
|
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|
Results are medians, with ranges in parentheses. Concentrations of mediators were determined in the culture supernatant of the fibroblast-like synoviocytes (FLS) by ELISA as described in the Materials and methods section. FLS were incubated for 24 hours with 1 ml of culture medium containing 1% FCS (negative control), 975 μl of culture medium supplemented with either (1) 25 μl of interleukin (IL)-1β (final concentration 125 pg/ml; positive control), (2) 25 μl (onefold (1×) or threefold (3×) concentrated) microparticles (MP), or (3) MP-free synovial fluid. P*, positive versus negative control; P†, MP (1×) versus MP-free synovial fluid; P‡, MP (3×) versus MP (1×). Nx/Nt, number of individual culture supernatants that contained elevated or decreased concentrations of mediators after incubation for 24 hours with isolated MP compared with MP-free synovial fluid, divided by the number of patients studied. GM-CSF, granulocyte/macrophage colony-stimulating factor; sICAM-1, soluble intracellular adhesion molecule-1; MCP-1, monocyte chemoattractant protein-1; VEGF, vascular endothelial growth factor. |
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|
Berckmans et al. Arthritis Research & Therapy 2005 7:R536 doi:10.1186/ar1706 |
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