Regulation of apoptosis-related proteins in sodium nitroprusside (SNP)-treated chondrocytes. Chondrocytes were treated with 0.1 mM SNP 14 hours prior to being treated with 1 mM SNP, and the expressions of Mcl-1, Bcl-XL, and p53 were analyzed after 4 hours by western blot. Protein was extracted from chondrocytes and 20 μg each protein sample was separated by 12% SDS-PAGE and blotted with respective antibodies. Data are representative of five samples from different donors.
Kim et al. Arthritis Research & Therapy 2005 7:R526 doi:10.1186/ar1705