Figure 1.

Verification of citrullination of collagen type II (CII) and rat serum albumin (RSA) by immunoblotting. Using the recombinant anti-citrullinated protein antibody RA3, citrulline was detected in the citrullinated CII (Cit-CII) sample (upper left) as well as in the citrullinated RSA (Cit-RSA) sample (upper right), while uncitrullinated CII (lower left) and uncitrullinated RSA (lower right) were negative for citrulline.