Figure 6.

Early detection (30 hours after KRN serum transfer) of the inflammatory joint by NIR2-folate. (a) White-light image showed no remarkable swelling at bilateral paws. (b) Merged near-infrared fluorescent signal with a white-light image showed increase fluorescence signal intensity at the dorsal aspect of the right wrist, which has a 1.8-fold increase compared with the left wrist. (c) H&E-stain histology of the right wrist showed polymorphonuclear cell infiltration at the dorsal aspect of the right wrist (arrow). Magnification, 20 × (400 ×, insert). (d) Histology of the left wrist showed no remarkable inflammatory cell infiltration. Magnification, 20 × (400 ×, insert). (e) Immunohistochemistry of the right wrist showed Mac-3-positive cell infiltration at subsynovial tissues. Magnification, 400 ×.