Figure 4.

Colocalization of Mac-3-positive cells and NIR2-folate uptake cells in a lipopolysaccharide-induced arthritic ankle 48 hours after NIR2-folate injection. (a) Immunofluorescence staining for activated macrophage revealed in the FITC channel. (b) NIR2-folate uptake cells are revealed in the near-infrared fluorescent channel. (c) Superimposed image shows Mac-3-positive cells colocalized well with NIR2-folate cellular origins. (d) A negative control without primary antibody. Original magnification, 400 ×.