Depletion of CD25-expressing cells by anti-CD25 treatment. Mice immunized with methylated bovine serum albumin (mBSA) were injected intraperitoneally with 0.5 mg PC61 (anti-CD25) or rat IgG as control 4 and 2 days before arthritis induction. Representative example for flow-cytometric assessment of depletion in spleen cells, using a non-cross-reactive anti-CD25 antibody (7D4) at the time of arthritis induction (day 0).
Frey et al. Arthritis Res Ther 2005 7:R291 doi:10.1186/ar1484