Table 1

Sequences of primers and probes for quantitative real-time polymerase chain reaction

Gene
GenBank accession no.
Primers (5'→3')
Conc. (nM)
Probe (5'→3')
MgCl2 (mM)

GAPDH
NM_002046
Forward: GAAGGTGAAGGTCGGAGTC
50
CAAGCTTCCCGTTCTCAGCC
5.5


Reverse: GAAGATGGTGATGGGATTTC
900


TOB1
NM_005749
Forward: TCTGCTGCTGTAAGCCCTACCT
300
CGGTCCACTCAGCCTTTAACCTTTACCACT
6.5


Reverse: TTCATTTTGGTAGAGCCGAACTT
900


Ki-67
NM_002417
Forward: CAGTGATCAACGCCGTAGGTC
900
CTTCCAGCAGCAAATCTCAGACAGAGGTTC
6.0


Reverse: TCGGCTGATAGACACTCTCTTTTG
900


COL2A1
NM_001844
Forward:CAACACTGCCAACGTCCAGAT
50
ACCTTCCTACGCCTGCTGTCCACG
5.5


Reverse: CTGCTTCGTCCAGATAGGCAAT
300


MMP-13
NM_002427
Forward: TCCTCTTCTTGAGCTGGACTCATT
900
TCCTCAGACAAATCATCTTCATCACCACCAC
7.0


Reverse: CGCTCTGCAAACTGGAGGTC
50



GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MMP, matrix metalloproteinase; COL2A1, collagen type II (alpha 1 chain); TOB1, Transducer of ERBB2.

Gebauer et al. Arthritis Res Ther 2005 7:R274   doi:10.1186/ar1479