Table 1 |
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|
Sequences of primers and probes for quantitative real-time polymerase chain reaction |
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|
Gene |
GenBank accession no. |
Primers (5'→3') |
Conc. (nM) |
Probe (5'→3') |
MgCl2 (mM) |
|
|
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|
GAPDH |
NM_002046 |
Forward: GAAGGTGAAGGTCGGAGTC |
50 |
CAAGCTTCCCGTTCTCAGCC |
5.5 |
|
Reverse: GAAGATGGTGATGGGATTTC |
900 |
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|
TOB1 |
NM_005749 |
Forward: TCTGCTGCTGTAAGCCCTACCT |
300 |
CGGTCCACTCAGCCTTTAACCTTTACCACT |
6.5 |
|
Reverse: TTCATTTTGGTAGAGCCGAACTT |
900 |
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|
Ki-67 |
NM_002417 |
Forward: CAGTGATCAACGCCGTAGGTC |
900 |
CTTCCAGCAGCAAATCTCAGACAGAGGTTC |
6.0 |
|
Reverse: TCGGCTGATAGACACTCTCTTTTG |
900 |
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|
COL2A1 |
NM_001844 |
Forward:CAACACTGCCAACGTCCAGAT |
50 |
ACCTTCCTACGCCTGCTGTCCACG |
5.5 |
|
Reverse: CTGCTTCGTCCAGATAGGCAAT |
300 |
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|
MMP-13 |
NM_002427 |
Forward: TCCTCTTCTTGAGCTGGACTCATT |
900 |
TCCTCAGACAAATCATCTTCATCACCACCAC |
7.0 |
|
Reverse: CGCTCTGCAAACTGGAGGTC |
50 |
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|
|
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|
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MMP, matrix metalloproteinase; COL2A1, collagen type II (alpha 1 chain); TOB1, Transducer of ERBB2. |
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|
Gebauer et al. Arthritis Res Ther 2005 7:R274 doi:10.1186/ar1479 |
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