Figure 2.

Effects of various glycosaminoglycans (GAGs) on antithrombin (AT). Hyaluronic acid (HA), chondroitin sulfate A (CSA), chondroitin sulfate B (CSB), chondroitin sulfate C (CSC), keratan sulfate (KS), heparin, or heparan (500 μg/ml) was incubated with 150 μg/ml antithrombin and 5 mmol/l CaCl₂. Controls consisted of only GAG or AT and blank (working buffer only). Thrombin activity of blank was considered as 1 and the activities of other tests were normalized based on comparisons with blank. Values are expressed as mean ± standard deviation of data from triplicate experiments.