Table 1 |
|
|
Specificity of [3H]NECA binding to A2B adenosine receptors in control neutrophil membranes |
|
| [3H]NECA Ki (μmol/l) |
|
|
|
|
| NECA |
0.315 ± 0.028 |
| 2 Cl-adenosine |
0.954 ± 0.600 |
| R-PIA |
1000 ± 86 |
| SCH58261 |
>10 |
| Teofilline |
47 ± 3.5 |
| MRS1706 |
0.005 ± 0.0003 |
| DPCPX |
2 ± 0.12 |
| MRS1220 |
>10 |
|
|
|
|
Competition experiments were performed, incubating aliquots of neutrophil membranes with 20 nmol/l [3H]NECA (plus 50 nmol/l CPA and 100 nmol/l SCH58261) in the presence of increasing ligand concentrations. Ki values are expressed as mean ± SEM of three separate experiments. Ki values were calculated from IC50 values (concentration of drug causing 50% inhibition of specific binding) using the Cheng and Prusoff equation. |
|
|
Bazzichi et al. Arthritis Res Ther 2004 7:R189 doi:10.1186/ar1468 |
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