Figure 4.

Cadherin-11 mediates lining layer-like formation. Discrete regions of tissue culture dishes were coated with fibronectin (FN), followed by blocking of the entire dish with bovine serum albumin (BSA). L-cells were seeded at equal numbers and cultured under serum-free conditions. After 2 days in culture, cadherin-11-expressing L-cells condensed and accumulated at the FN–BSA interphase to form a lining layer-like structure. In contrast, vector control L-cells were randomly distributed at the FN-coated area and did not form a lining layer. (Reproduced from The Journal of Experimental Medicine 2004, 200:1678 by permission of The Rockefeller University Press [16].)