Table 1 |
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TaqMan® probes and primers for the genes of interest |
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| Gene of interest |
Forward primer |
Reverse primer |
Probe |
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| IL-1β |
5'-CACCTCTCAAGCAGAGCACAG |
5'-GGGTTCCATGGTGAAGTCAAC |
5'-6-FAM-TGTCCCGACCATTGCTGTTTCCTAGG-TAMRA |
| IL-6 |
5'-CAAGACCATCCAACTCATCTTG |
5'-CACAGTGAGGAATGTCCACAAAC |
5'-6-FAM-TCGGCAAACCTAGTGTGCTATGCCTAAGCA-TAMRA |
| TNF-α |
5'-CCAGGTTCTCTTCAAGGGACAA |
5'-CTCCTGGTATGAAATGGCAAATC |
5'-6-FAM-CCCGACTATGTGCTCCTCACCCACA-TAMRA |
| GRO1 |
5'-TGTGTTGAAGCTTCCCTTGGA |
5'-TGAGACGAGAAGGAGCATTGGT |
5'-6-FAM-TGTCTAGTTTGTAGGGCACAATGCCCT-TAMRA |
| CD14 |
5'-GGACGAGGAAAGTGTCCGCT |
5'-AGGTACTCCAGGCTGCGACC |
5'-6-FAM-TTCTATGCGCGGGGGCGGAA-TAMRA |
| CD3 |
5'-GGATGGAGTTCGCCAGTCAA |
5'-GGTTTCCTTGGAGACGGCTG |
5'-6-FAM-ACAGGTCTACCAGCCCCTCAAGGACCG-TAMRA |
| Ubiquitin |
5'-CGAGAACGTGAAGGCCAAGA |
5'-GGAGGACAAGGTGCAGGGTT |
5'-6-FAM-CCCCTGACCAGCAGAGGCTCATCTTTG-TAMRA |
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IL, interleukin; TNF, tumour necrosis factor. |
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Rioja et al. Arthritis Res Ther 2005 7:R101 doi:10.1186/ar1458 |
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