Relaxin induces collagenase-1 and stromelysin-1 in pubic symphysis fibrocartilage but not in articular cartilage. Pubic symphysis fibrocartilage or knee articular cartilage explants were exposed for 48 hours to basal control medium (Ct), β-estradiol (Es, 20 ng/ml), or relaxin (R, 0.1 ng/ml) or to β-estradiol plus relaxin (Es+R). Conditioned medium, standardized by tissue weight, was subjected to western blotting for collagenase-1 (a, b) or stromelysin-1 (c, d). Cl-1, collagenase-1; Sl-1, stromelysin-1.
Naqvi et al. Arthritis Res Ther 2005 7:R1-R11 doi:10.1186/ar1451